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General expression vectors for Staphylococcus carnosus enabled efficient production of the outer membrane protein A of Klebsiella pneumoniae
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0002-9282-0174
2002 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 210, no 2, 263-270 p.Article in journal (Refereed) Published
Abstract [en]

General expression vectors, designed for intracellular expression or secretion of recombinant proteins in the non-pathogenic Staphylococcus carnosus, were constructed. Both vector systems encode two different affinity tags, an upstream albumin binding protein and a downstream hexahistidyl peptide, and are furnished with cleavage sites for two site-specific proteases for optional affinity tag removal. To evaluate the novel vectors, the gene encoding the outer membrane protein A (OmpA) of Klebsiella pneumoniae was introduced into the vectors. Efficient production was demonstrated in both systems, although, as expected for OmpA fusions, somewhat better intracellularly, and the fusion proteins could be recovered as full-length products by affinity chromatography.

Place, publisher, year, edition, pages
2002. Vol. 210, no 2, 263-270 p.
Keyword [en]
albumin binding protein, intracellular expression, outer membrane protein A, secretion, Klebsiella pneumoniae, Staphylococcus carnosus, recombinant subunit vaccines, escherichia-coli, surface display, hyicus lipase, proteolytic degradation, fusion protein, system, purification, secretion, bacteria
URN: urn:nbn:se:kth:diva-21620ISI: 000176246200016OAI: diva2:340318
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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