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Engineering of staphylococcal surfaces for biotechnological applications
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0002-9282-0174
2002 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 96, no 1, 67-78 p.Article, review/survey (Refereed) Published
Abstract [en]

Novel surface proteins can be introduced onto bacterial cell surfaces by recombinant means. Here, we describe various applications of two such display systems for the food-grade bacteria Staphylococcus carnosus and Staphylococcus xylosus, respectively. The achievements in the use of such staphylococci as live bacterial vaccine delivery vehicles will be described. Co-display of proteins and peptides with adhesive properties to enable targeting of the bacteria, have significantly improved the vaccine delivery potential. Recently, protective immunity to respiratory syncytial virus (RSV) could be evoked in mice by intranasal immunization using such 'second generation' vaccine delivery systems. Furthermore, antibody fragments and other 'affinity proteins' with capacity to specifically bind a certain protein, e.g. Staphylococcus aureus protein A-based affibodies, have been surface-displayed on staphylococci as initial efforts to create whole-cell diagnostic devices. Surface display of metal-binding peptides, or protein domains into which metal binding properties has been engineered by combinatorial protein engineering, have been exploited to create staphylococcal bioadsorbents for potential environmental or biosensor applications. The use of these staphylococcal surface display systems as alternatives for display of large protein libraries and subsequent affinity selection of relevant binding proteins by fluorescence-activated cell sorting (FACS) will be discussed.

Place, publisher, year, edition, pages
2002. Vol. 96, no 1, 67-78 p.
Keyword [en]
bioadsorbent, biosensor, diagnostic device, environmental application, Staphylococcus carnosus, Staphylococcus xylosus, surface display, vaccine delivery, escherichia-coli-cells, cellulose-binding domain, toxin-b subunit, combinatorial libraries, intranasal immunization, recombinant proteins, antibody-responses, vaccine delivery, heavy-metals, display
National Category
Biological Sciences
URN: urn:nbn:se:kth:diva-21628DOI: 10.1016/S0168-1656(02)00038-XISI: 000176318300008PubMedID: 12142144OAI: diva2:340326
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2010-09-13Bibliographically approved

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Wernérus, HenrikLehtiö, JanneSamuelson, PatrikStåhl, Stefan
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