SNP typing by apyrase-mediated allele-specific primer extension on DNA microarrays
2002 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 30, no 15Article in journal (Refereed) Published
This study reports the development of a microarray-based allele-specific extension method for typing of single nucleotide polymorphisms (SNPs). The use of allele-specific primers has been employed previously to identify single base variation but it is acknowledged that certain mismatches are not refractory to extension. Here we have overcome this limitation by introducing apyrase, a nucleotide-degrading enzyme, to the extension reaction. We have show previously that DNA polymerases exhibit slower reaction kinetics when extending a mismatched primer compared with a matched primer. This kinetic difference is exploited in the apyrase-mediated allele-specific extension (AMASE) assay, allowing incorporation of nucleotides when the reaction kinetics are fast but degrading the nucleotides before extension when the reaction kinetics are slow. Here we show that five homozygous variants (14% of the total number of variants) that were incorrectly scored in the absence of apyrase were correctly typed when apyrase was included in the extension reaction. AMASE was performed in situ on the oligonucleotide microarrarys using fluorescent nucleotides to type 10 SNPs and two indels in 17 individuals generating approximately 200 genotypes. Cluster analysis of these data shows three distinct clusters with clear-cut boundaries. We conclude that SNP typing on oligonucleotide microarrays by AMASE is an efficient, rapid and accurate technique for large-scale genotyping.
Place, publisher, year, edition, pages
2002. Vol. 30, no 15
single nucleotide polymorphisms, polymerase-chain-reaction, oligonucleotide arrays, amplification, gene, pcr, discrimination, fluorescence, mutations, markers
IdentifiersURN: urn:nbn:se:kth:diva-21795ISI: 000177325100024OAI: oai:DiVA.org:kth-21795DiVA: diva2:340493
QC 201005252010-08-102010-08-10Bibliographically approved