Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
beta-Adrenoceptor agonist sensitizes the dopamine-1 receptor in renal tubular cells
2002 (English)In: Acta Physiologica Scandinavica, ISSN 0001-6772, E-ISSN 1365-201X, Vol. 175, no 4, 333-340 p.Article in journal (Refereed) Published
Abstract [en]

The renal effects of dopamine are mainly mediated via the dopamine-1 receptor (D1 receptor). This receptor is recruited from intracellular compartments to the plasma membrane by dopamine and atrial natriuretic peptide (ANP), via adenylyl cyclase activation. We have studied whether isoproterenol, a beta -adrenoceptor (beta -AR) agonist that may interact with dopamine in the regulation of rat renal Na+, K+ -adenosine triphosphatase (ATPase) activity, can recruit D1 receptors to the plasma membrane. The spatial regulation of D1 receptors was examined using confocal microscopy techniques in LLCPK cells and the functional interaction between dopamine and isoproterenol was examined by studying their effects on Na+, K+ -ATPase activity in microdissected single proximal tubular segments from rat. Isoproterenol was found to translocate the D1 receptors from the interior of the cell towards the plasma membrane. The recruitment of dopamine 1 receptors was found to be cyclic adenosine phosphate (cAMP) dependent, while protein kinase C (PKC) activation was not involved. The functional studies on Na+, K+ -ATPase activity showed that the effect of isoproterenol was abolished by a D1-like receptor antagonist (SCH 23390), and mediated via protein kinase A (PKA) and PKC dependent pathways. The results provide an explanation for the interaction between G protein-coupled receptors. The effects of isoproterenol on Na+, K+ -ATPase activity can be explained by a heterologous recruitment of D1 receptors to the plasma membrane.

Place, publisher, year, edition, pages
2002. Vol. 175, no 4, 333-340 p.
Keyword [en]
G-protein-coupled receptors, heterologous sensitization, isoproterenol, Na, K+-ATPase, receptor recruitment, na+-k+-atpase, protein-coupled receptors, intrarenal dopamine, inhibition, na+,k+-atpase, natriuresis, recruitment, segments
Identifiers
URN: urn:nbn:se:kth:diva-21807ISI: 000177361500008OAI: oai:DiVA.org:kth-21807DiVA: diva2:340505
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

Open Access in DiVA

No full text

Authority records BETA

Brismar, Hjalmar

Search in DiVA

By author/editor
Brismar, Hjalmar
In the same journal
Acta Physiologica Scandinavica

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 28 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf