Peptide-mediated delivery of green fluorescent protein into yeasts and bacteria
2002 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 215, no 2, 267-272 p.Article in journal (Refereed) Published
Stringent microbial cell barriers limit the application of many substances in research and therapeutics. Carrier peptides that penetrate or translocate across cell membranes may help overcome this problem. To assess peptide-mediated delivery into two yeast and three bacterial species, a range of cell penetrating and signal peptide sequences were fused to green fluorescent protein (GFP), expressed in Escherichia coli, partially purified and incubated with growing cells. Fluorescence microscopy indicated several peptides that mediated delivery. In particular, VLTNENPFSDP efficiently delivered GFP into Candida albicans and Staphylococcus aureus, while YKKSNNPFSD was most efficient for Bacillus subtilis and CFFKDEL for Escherichia coli. Carrier peptides may improve delivery of certain large molecular mass molecules into microorganisms for research and therapeutic applications.
Place, publisher, year, edition, pages
2002. Vol. 215, no 2, 267-272 p.
signal peptide, Candida albicans, Saccharomyces cerevisiae, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, eh domains, in-vivo, endocytosis, cells, recognition, membranes, blockers, receptor
IdentifiersURN: urn:nbn:se:kth:diva-22008ISI: 000178923300015OAI: oai:DiVA.org:kth-22008DiVA: diva2:340706
QC 201005252010-08-102010-08-10Bibliographically approved