Change search
ReferencesLink to record
Permanent link

Direct link
The mutagenic effect of ultraviolet-A1 on human skin demonstrated by sequencing the p53 gene in single keratinocytes
KTH, Superseded Departments, Biotechnology.
Show others and affiliations
2002 (English)In: Photodermatology, Photoimmunology & Photomedicine, ISSN 0905-4383, E-ISSN 1600-0781, Vol. 18, no 6, 287-293 p.Article in journal (Refereed) Published
Abstract [en]

Background. Sun exposure is accepted as the major risk factor for developing skin cancer, the most common cancer in the western world. Ultraviolet-B (UV-B) radiation is considered the causative agent, but recently several findings suggest a role also for ultraviolet-A (UV-A) radiation. Repeated suberythemal doses of ultraviolet-A1 (UV-A1) on healthy human skin induce an increase of p53 immunoreactive cells in epidermis, which may indicate cell cycle arrest and/or occurrence of p53 mutations. Methods: We have investigated the possible mutagenic effect of UV-A1 on skin by sequencing exons 4-11 and adjacent intron sequence of the p53 gene in immunoreactive single cells from three healthy individuals. Previously unexposed buttock skin was irradiated three times a week for 2 weeks with physiological fluences (40 J/cm(2)) of UV-A1. Punch biopsies were taken before and at different time-points after the exposure, and from these single p53 immunoreactive cells were isolated by using laser-assisted microdissection. Results: Three mutations - all being indicative of oxidative damage and most likely related to UV-A exposure were found among the 37 single cells from exposed skin, whereas no mutations were found in the 22 single cells taken before exposure. Conclusions: The findings indicate a mutagenic effect of low-dose UV-A1 on healthy human skin, which further demonstrates the importance of considering UV-A when taking protective measures against skin cancer.

Place, publisher, year, edition, pages
2002. Vol. 18, no 6, 287-293 p.
Keyword [en]
mutations, p53, single cells, UV-A1, dna-damage, visible radiation, base damage, cells, uva, expression, mutations, cancer, light, carcinogenesis
URN: urn:nbn:se:kth:diva-22098ISI: 000179548600003OAI: diva2:340796
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Lundeberg, Joakim
By organisation
In the same journal
Photodermatology, Photoimmunology & Photomedicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 24 hits
ReferencesLink to record
Permanent link

Direct link