The mutagenic effect of ultraviolet-A1 on human skin demonstrated by sequencing the p53 gene in single keratinocytes
2002 (English)In: Photodermatology, Photoimmunology & Photomedicine, ISSN 0905-4383, E-ISSN 1600-0781, Vol. 18, no 6, 287-293 p.Article in journal (Refereed) Published
Background. Sun exposure is accepted as the major risk factor for developing skin cancer, the most common cancer in the western world. Ultraviolet-B (UV-B) radiation is considered the causative agent, but recently several findings suggest a role also for ultraviolet-A (UV-A) radiation. Repeated suberythemal doses of ultraviolet-A1 (UV-A1) on healthy human skin induce an increase of p53 immunoreactive cells in epidermis, which may indicate cell cycle arrest and/or occurrence of p53 mutations. Methods: We have investigated the possible mutagenic effect of UV-A1 on skin by sequencing exons 4-11 and adjacent intron sequence of the p53 gene in immunoreactive single cells from three healthy individuals. Previously unexposed buttock skin was irradiated three times a week for 2 weeks with physiological fluences (40 J/cm(2)) of UV-A1. Punch biopsies were taken before and at different time-points after the exposure, and from these single p53 immunoreactive cells were isolated by using laser-assisted microdissection. Results: Three mutations - all being indicative of oxidative damage and most likely related to UV-A exposure were found among the 37 single cells from exposed skin, whereas no mutations were found in the 22 single cells taken before exposure. Conclusions: The findings indicate a mutagenic effect of low-dose UV-A1 on healthy human skin, which further demonstrates the importance of considering UV-A when taking protective measures against skin cancer.
Place, publisher, year, edition, pages
2002. Vol. 18, no 6, 287-293 p.
mutations, p53, single cells, UV-A1, dna-damage, visible radiation, base damage, cells, uva, expression, mutations, cancer, light, carcinogenesis
IdentifiersURN: urn:nbn:se:kth:diva-22098ISI: 000179548600003OAI: oai:DiVA.org:kth-22098DiVA: diva2:340796
QC 201005252010-08-102010-08-10Bibliographically approved