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Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging
KTH, Superseded Departments, Physics.
2003 (English)In: Journal of the Optical Society of America A, ISSN 0740-3232, Vol. 20, no 2, 368-379 p.Article in journal (Refereed) Published
Abstract [en]

We deduce the signal-to-noise ratio for fluorescence lifetime imaging when using frequency-domain methods. We assume mono-exponential decay and quantum-noise-limited performance. The results are compared with Monte Carlo simulations with good agreement. We also compare our results with previous investigations of time-domain methods for fluorescence lifetime imaging. For a given number of detected photons, we find that frequency-domain and time-domain methods are equally good. The correct choice of detection technique and its parameters is important for obtaining good results.

Place, publisher, year, edition, pages
2003. Vol. 20, no 2, 368-379 p.
Keyword [en]
time-resolved identification, multiple fluorophores, microscopy, molecules, laser, cells, ph
Identifiers
URN: urn:nbn:se:kth:diva-22209ISI: 000180643200018OAI: oai:DiVA.org:kth-22209DiVA: diva2:340907
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

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