Change search
ReferencesLink to record
Permanent link

Direct link
Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides
KTH, Superseded Departments, Biotechnology.
Show others and affiliations
2003 (English)In: Acta Crystallographica Section D: Biological Crystallography, ISSN 0907-4449, E-ISSN 1399-0047, Vol. 59, 535-537 p.Article in journal (Refereed) Published
Abstract [en]

Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 Angstrom) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 Angstrom).

Place, publisher, year, edition, pages
2003. Vol. 59, 535-537 p.
Keyword [en]
crystal-structure, arabidopsis tch4, wall
URN: urn:nbn:se:kth:diva-22331ISI: 000181609800023OAI: diva2:341029
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Brumer, HarryTeeri, Tuula T.
By organisation
In the same journal
Acta Crystallographica Section D: Biological Crystallography

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 18 hits
ReferencesLink to record
Permanent link

Direct link