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Ultrasonic-trap-enhanced selectivity in capillary electrophoresis
KTH, Superseded Departments, Physics.
KTH, Superseded Departments, Physics.ORCID iD: 0000-0003-2723-6622
2003 (English)In: Ultrasonics, ISSN 0041-624X, E-ISSN 1874-9968, Vol. 41, no 4, 329-333 p.Article in journal (Refereed) Published
Abstract [en]

We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-muM diam capillary of the CE system. Competition between acoustic and viscous forces result in inflow separation of mum-diam spheres according to their size. Experiments separating 2.8- and 2.1-mum-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 10(4) compared to current CE systems. Theoretical calculations show room for further improvement.

Place, publisher, year, edition, pages
2003. Vol. 41, no 4, 329-333 p.
Keyword [en]
ultrasonic trapping, particle separation, capillary electrophoresis, concentration limit of detection, microparticles, fluorescence, droplet, protein, wave
Identifiers
URN: urn:nbn:se:kth:diva-22606DOI: 10.1016/s0041-624x(02)00460-2ISI: 000183679100015OAI: oai:DiVA.org:kth-22606DiVA: diva2:341304
Note
QC 20100525Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved

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Hertz, Hans M.

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