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Method for clone checking
KTH, Superseded Departments, Biochemistry and Biotechnology.
2003 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 24, no 11, 1712-1715 p.Article in journal (Refereed) Published
Abstract [en]

A new method for simple and fast clone checking is described. We combined the Pyrosequencing(TM) technology with a preprogrammed nucleoticle dispensation strategy for fast analysis of DNA constructs. To test this method, the N-terminus region of plasmids constructed for the production of recombinant apyrase was analyzed. Of the ten plasmids tested, seven constructs were correct, two constructs showed one base deletion, and one construct showed deletion of a 195 bp fragment. The preprogrammed nucleoticle dispensation strategy allowed the identification of the sequence downstream of the deletions. Thus, this method determines both the location and nature of possible artifacts.

Place, publisher, year, edition, pages
2003. Vol. 24, no 11, 1712-1715 p.
Keyword [en]
apyrase, clone checking, deletion, mutation, pyrosequencing technology
URN: urn:nbn:se:kth:diva-22607DOI: 10.1002/elps.200305434ISI: 000183682800003OAI: diva2:341305
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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