Change search
ReferencesLink to record
Permanent link

Direct link
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues
Show others and affiliations
2003 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 2, no 6, 405-414 p.Article in journal (Refereed) Published
Abstract [en]

Here we show that an affinity proteomics strategy using affinity-purified antibodies raised against recombinant human protein fragments can be used for chromosome-wide protein profiling. The approach is based on affinity reagents raised toward bioinformatics-designed protein epitope signature tags corresponding to unique regions of individual gene loci. The genes of human chromosome 21 identified by the genome efforts were investigated, and the success rates for de novo cloning, protein production, and antibody generation were 85, 76, and 56%, respectively. Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the putative gene products. The results suggest that this affinity proteomics strategy can be used to produce a proteome atlas, describing distribution and expression of proteins in normal tissues as well as in common cancers and other forms of diseased tissues.

Place, publisher, year, edition, pages
2003. Vol. 2, no 6, 405-414 p.
Keyword [en]
bacterial receptor domain, cdna-encoded proteins, mass-spectrometry, overlap extension, escherichia-coli, identification, expression, binding, human-chromosome-21, generation
URN: urn:nbn:se:kth:diva-22718DOI: 10.1074/mcp.M300022-MCP200ISI: 000184559300006OAI: diva2:341416
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Lundeberg, JoakimStåhl, StefanUhlén, Mathias
By organisation
In the same journal
Molecular & Cellular Proteomics

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 74 hits
ReferencesLink to record
Permanent link

Direct link