Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues
2003 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 2, no 6, 405-414 p.Article in journal (Refereed) Published
Here we show that an affinity proteomics strategy using affinity-purified antibodies raised against recombinant human protein fragments can be used for chromosome-wide protein profiling. The approach is based on affinity reagents raised toward bioinformatics-designed protein epitope signature tags corresponding to unique regions of individual gene loci. The genes of human chromosome 21 identified by the genome efforts were investigated, and the success rates for de novo cloning, protein production, and antibody generation were 85, 76, and 56%, respectively. Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the putative gene products. The results suggest that this affinity proteomics strategy can be used to produce a proteome atlas, describing distribution and expression of proteins in normal tissues as well as in common cancers and other forms of diseased tissues.
Place, publisher, year, edition, pages
2003. Vol. 2, no 6, 405-414 p.
bacterial receptor domain, cdna-encoded proteins, mass-spectrometry, overlap extension, escherichia-coli, identification, expression, binding, human-chromosome-21, generation
IdentifiersURN: urn:nbn:se:kth:diva-22718DOI: 10.1074/mcp.M300022-MCP200ISI: 000184559300006OAI: oai:DiVA.org:kth-22718DiVA: diva2:341416
QC 201005252010-08-102010-08-10Bibliographically approved