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Engineered enzymes for improved organic synthesis
KTH, Superseded Departments, Biochemistry and Biotechnology.
KTH, Superseded Departments, Biochemistry and Biotechnology.ORCID iD: 0000-0002-9577-832X
2003 (English)In: Current Opinion in Biotechnology, ISSN 0958-1669, E-ISSN 1879-0429, Vol. 14, no 4, 395-400 p.Article, review/survey (Refereed) Published
Abstract [en]

Recent developments to modify enzymes for use in organic synthesis have targeted several areas. These include altering the reaction mechanism of the enzyme to catalyse new reactions, switching substrate specificity, expanding substrate specificity, and improving substrate specificity, such as enantioselectivity in kinetic resolutions. Such modifications can be achieved either by rational redesign, which requires knowledge of the enzyme structure, or by random mutagenesis methods followed by screening. Both strategies of enzyme engineering can be successful and are very useful for improving the utility of enzymes for applied catalysis. Several examples illustrating these concepts in a variety of enzyme classes have appeared recently.

Place, publisher, year, edition, pages
2003. Vol. 14, no 4, 395-400 p.
Keyword [en]
directed evolution, mutant glycosidases, selective enzymes, rational redesign, substrate, phosphotriesterase, enantioselectivity, lipase, combinatorial, aldolase
URN: urn:nbn:se:kth:diva-22787DOI: 10.1016/s0958-1669(03)00095-8ISI: 000185140300007OAI: diva2:341485
QC 20100525Available from: 2010-08-10 Created: 2010-08-10Bibliographically approved

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