Change search
ReferencesLink to record
Permanent link

Direct link
A transcriptional timetable of autumn senescence
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.
Show others and affiliations
2004 (English)In: Genome Biology, ISSN 1465-6906, E-ISSN 1465-6914, Vol. 5, no 4, R24- p.Article in journal (Refereed) Published
Abstract [en]

Background: We have developed genomic tools to allow the genus Populus ( aspens and cottonwoods) to be exploited as a full-featured model for investigating fundamental aspects of tree biology. We have undertaken large-scale expressed sequence tag ( EST) sequencing programs and created Populus microarrays with significant gene coverage. One of the important aspects of plant biology that cannot be studied in annual plants is the gene activity involved in the induction of autumn leaf senescence. Results: On the basis of 36,354 Populus ESTs, obtained from seven cDNA libraries, we have created a DNA microarray consisting of 13,490 clones, spotted in duplicate. Of these clones, 12,376 (92%) were confirmed by resequencing and all sequences were annotated and functionally classified. Here we have used the microarray to study transcript abundance in leaves of a free-growing aspen tree ( Populus tremula) in northern Sweden during natural autumn senescence. Of the 13,490 spotted clones, 3,792 represented genes with significant expression in all leaf samples from the seven studied dates. Conclusions: We observed a major shift in gene expression, coinciding with massive chlorophyll degradation, that reflected a shift from photosynthetic competence to energy generation by mitochondrial respiration, oxidation of fatty acids and nutrient mobilization. Autumn senescence had much in common with senescence in annual plants; for example many proteases were induced. We also found evidence for increased transcriptional activity before the appearance of visible signs of senescence, presumably preparing the leaf for degradation of its components.

Place, publisher, year, edition, pages
2004. Vol. 5, no 4, R24- p.
Keyword [en]
cytosolic glutamine-synthetase, programmed cell-death, leaf senescence, gene-expression, leaves, proteins, genomics, biology, plants, aspen
National Category
Industrial Biotechnology
URN: urn:nbn:se:kth:diva-23293DOI: 10.1186/gb-2004-5-4-r24ISI: 000220584700010PubMedID: 15059257ScopusID: 2-s2.0-1842619264OAI: diva2:341991
QC 20100525 QC 20111102Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2012-02-24Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Andersson, AndersBhalerao, RupaliSterky, FredrikAspeborg, HenrikLundeberg, JoakimUhlén, MathiasNilsson, Peter
By organisation
In the same journal
Genome Biology
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 72 hits
ReferencesLink to record
Permanent link

Direct link