Dynamic combinatorial carbohydrate libraries: Probing the binding site of the concanavalin A lectin
2004 (English)In: Chemistry - A European Journal, ISSN 0947-6539, E-ISSN 1521-3765, Vol. 10, no 7, 1711-1715 p.Article in journal (Refereed) Published
Dynamic combinatorial chemistry (DCC) has emerged as an efficient approach to receptor/ligand identification based on the generation of combinatorial libraries by reversible interconversion of the library constituents. In this study, the implementation of such libraries on carbohydrate-lectin interactions was examined with the plant lectin Concanavalin A as a target species. Dynamic carbohydrate libraries were generated from a pool of carbohydrate aldehydes and hydrazide linker/scaffold components through re- versible acylhydrazone exchange, resulting in libraries containing up to 474 constituents. Dynamic deconvolution allowed the efficient identification of the structural features required for binding to Concanavalin A and the selection of a strong binder, a tritopic mannoside, showing an IC50-value of 22 mum.
Place, publisher, year, edition, pages
2004. Vol. 10, no 7, 1711-1715 p.
carbohydrates, combinatorial chemistry, concanavalin A, glycoconjugates, receptors, galnac-specific lectins, molecular recognition, chemical evolution, generation, amplification, inhibitors, chemistry, helicate, dendrimers, component
IdentifiersURN: urn:nbn:se:kth:diva-23323DOI: 10.1002/chem.200305551ISI: 000220771700014ScopusID: 2-s2.0-1842784768OAI: oai:DiVA.org:kth-23323DiVA: diva2:342021
QC 201005252010-08-102010-08-102011-10-31Bibliographically approved