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Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous termination
Department of Molecular Evolution, Evolutionary Biology Center, Uppsala University.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0002-3627-6899
Danish Archaea Center, Institute of Molecular Biology, University of Copenhagen.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0002-4657-8532
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2004 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 101, no 18, 7046-7051 p.Article in journal (Refereed) Published
Abstract [en]

Chromosome replication origins were mapped in vivo in the two hyperthermophilic archaea, Sulfolobus acidocaidarius and Sulfolobus solfataricus, by using microarray-based marker frequency analysis. Bidirectional replication was found to be initiated in near synchrony from three separate sites in both organisms. Two of the three replication origins in each species were located in the vicinity of a cdc6/orc1 replication initiation gene, whereas no known replication-associated gene could be identified near the third origin in either organism. In contrast to initiation, replication termination occurred asynchronously, such that certain replication forks continued to progress for >40 min after the others had terminated. In each species, all replication forks advanced at similar DNA polymerization rates; this was found to be an order of magnitude below that displayed by Escherichia coli and thus closer to eukaryotic elongation rates. In S. acidocaidarius, a region containing short regularly spaced repeats was found to hybridize aberrantly, as compared to the rest of the chromosome, raising the possibility of a centromere-like function.

Place, publisher, year, edition, pages
2004. Vol. 101, no 18, 7046-7051 p.
Keyword [en]
thermophilic archaea, dna, genus, acidocaldarius, temperature
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-23389DOI: 10.1073/pnas.0400656101ISI: 000221265000039Scopus ID: 2-s2.0-2342468069OAI: oai:DiVA.org:kth-23389DiVA: diva2:342087
Note
QC 20100830Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Microarray-based investigation of genome and transcriptome organisation in the archaeon sulfolobus
Open this publication in new window or tab >>Microarray-based investigation of genome and transcriptome organisation in the archaeon sulfolobus
2005 (English)Doctoral thesis, comprehensive summary (Other scientific)
Place, publisher, year, edition, pages
Stockholm: KTH, 2005. 47 p.
Keyword
Biotechnology, microarray, Sulfolobus, gene expression, archaea, mRNA decay, genome evolution, Bioteknik
National Category
Industrial Biotechnology
Identifiers
urn:nbn:se:kth:diva-142 (URN)91-7283-979-1 (ISBN)
Public defence
2005-03-11, Sal D1, Lindstedtsvägen 17, Stockholm, 10:00
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Note
QC 20100830Available from: 2005-03-03 Created: 2005-03-03 Last updated: 2010-08-30Bibliographically approved

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