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Ultrasonic enrichment of microspheres for ultrasensitive biomedical analysis in confocal laser-scanning fluorescence detection
KTH, Superseded Departments, Physics.
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2004 (English)In: Journal of Applied Physics, ISSN 0021-8979, E-ISSN 1089-7550, Vol. 96, no 2, 1242-1248 p.Article in journal (Refereed) Published
Abstract [en]

An ultrasonic particle concentrator based on a standing-wave hemispherical resonator is combined with confocal laser-scanning fluorescence detection. The goal is to perform ultrasensitive biomedical analysis by concentration of biologically active microspheres. The standing-wave resonator consists of a 4 MHz focusing ultrasonic transducer combined with the optically transparent plastic bottom of a disposable 96-well microplate platform. The ultrasonic particle concentrator collects suspended microspheres into dense, single-layer aggregates at well-defined positions in the sample vessel of the microplate, and the fluorescence from the aggregates is detected by the confocal laser-scanning system. The biochemical properties of the system are investigated using a microsphere-based human thyroid stimulating hormone assay.

Place, publisher, year, edition, pages
2004. Vol. 96, no 2, 1242-1248 p.
Keyword [en]
microvolume assay technology, 2-photon excitation, suspended particles, separation, biotechnology, immunoassay
National Category
Physical Sciences
URN: urn:nbn:se:kth:diva-23550DOI: 10.1063/1.1763226ISI: 000222391500048ScopusID: 2-s2.0-3242667289OAI: diva2:342248
QC 20100525 QC 20110926Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2011-09-26Bibliographically approved

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Wiklund, MartinHertz, Hans M.
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