Heating caused by radiofrequency irradiation and sample rotation in C-13 magic angle spinning NMR studies of lipid membranes
2004 (English)In: Magnetic Resonance in Chemistry, ISSN 0749-1581, E-ISSN 1097-458X, Vol. 42, no 10, 875-881 p.Article in journal (Refereed) Published
Application of rapid sample rotation and radiofrequency irradiation in magic angle spinning (MAS) NMR of lipid bilayers can significantly increase the sample temperature. In this work, we studied the extent of heating during the acquisition of H-1-decoupled C-13 MAS spectra of hydrated dimyristoylphosphatidyl-choline (DMPC) in the L. phase. First, we describe a simple procedure for determining the increase in temperature by observing the shift of the H-1 water signal. The method is then used to identify and assess the various factors that contribute to the sample heating. The important factors discussed in this paper include: (i) the spinning speed, (ii) the variable-temperature gas pressure, (iii) the rotor geometry, (iv) the power, duration and frequency of the radiofrequency irradiation and (v) the hydration level. A comparison of different heteronuclear decoupling schemes in terms of their ability to produce highly resolved C-13 spectra of DMPC is also reported.
Place, publisher, year, edition, pages
2004. Vol. 42, no 10, 875-881 p.
NMR, C-13 NMR, H-1 NMR, magic angle spinning, cross-polarization magic angle spinning, magic angle spinning heating, radiofrequency heating, heteronuclear decoupling, lipid membrane samples, nuclear-magnetic-resonance, band decoupling sequence, solid-state nmr, liquid-crystals, temperature-gradients, cross-polarization, mas nmr, chemical-shift, biological-membranes, dipolar
IdentifiersURN: urn:nbn:se:kth:diva-23746DOI: 10.1002/mrc.1477ISI: 000224047800006OAI: oai:DiVA.org:kth-23746DiVA: diva2:342445
QC 201005252010-08-102010-08-10Bibliographically approved