Change search
ReferencesLink to record
Permanent link

Direct link
Mutation spectra of epidermal p53 clones adjacent to basal cell carcinoma and squamous cell carcinoma
KTH, Superseded Departments, Biotechnology.
Show others and affiliations
2004 (English)In: Experimental dermatology, ISSN 0906-6705, E-ISSN 1600-0625, Vol. 13, no 10, 643-650 p.Article in journal (Refereed) Published
Abstract [en]

Foci of normal keratinocytes overexpressing p53 protein are frequently found in normal human skin. Such epidermal p53 clones are common in chronically sun-exposed skin and have been suggested to play a role in skin cancer development. In the present study, we have analyzed the prevalence of p53 mutations in epidermal p53 clones from normal skin surrounding basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Using laser-assisted microdissection, 37 epidermal p53 clones adjacent to BCC (21) and SCC (16) were collected. Genetic analysis was performed using a multiplex/nested polymerase chain reaction followed by direct DNA sequencing of p53 exons 2-11. In total, 21 of 37 analyzed p53 clones consisted of p53-mutated keratinocytes. The identified mutations were located in p53 exons 4-8, corresponding to the sequence-specific DNA-binding domain. All mutations were missense, and 78% displayed a typical ultraviolet signature. The frequency of p53 mutations was similar in skin adjacent to BCC compared to SCC. The presented data confirm and extend previous knowledge on the genetic background of epidermal p53 clones. The mutation spectra found in epidermal p53 clones resemble that of non-melanoma skin cancer. Approximately, 40% of the epidermal p53 clones lacked an underlying p53 mutation, suggesting that other genetic events in genes up- or downstream of the p53 gene can generate foci of normal keratinocytes overexpressing p53 protein.

Place, publisher, year, edition, pages
2004. Vol. 13, no 10, 643-650 p.
Keyword [en]
epidermal p53 clone, BCC, SCC, mutation, microdissection, skin-cancer, codon-72 polymorphism, single cells, mutant p53, dna-repair, keratinocytes, gene, susceptibility, apoptosis, sunburn
National Category
Medical Biotechnology
URN: urn:nbn:se:kth:diva-23754DOI: 10.1111/j.0906-6705.2004.00211.xISI: 000224071000007ScopusID: 2-s2.0-5144232482OAI: diva2:342453
QC 20100525 QC 20110922Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2011-09-22Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Gustafsson, AnnaSivertsson, ÅsaLundeberg, Joakim
By organisation
In the same journal
Experimental dermatology
Medical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 31 hits
ReferencesLink to record
Permanent link

Direct link