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Parallel single molecule detection with a fully integrated single-photon 2X2 CMOS detector array
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2004 (English)In: Journal of Biomedical Optics, ISSN 1083-3668, Vol. 9, no 5, 913-921 p.Article in journal (Refereed) Published
Abstract [en]

We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2 X 2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an actively quenched avalanche photodiode (APD)] by measurements on free diffusing molecules at different concentrations. Despite the order of magnitude lower detection efficiency of the CMOS detector compared to the state-of-the-art single-photon detecting module, we achieve single molecule sensitivity and reliably determine molecule concentrations. In addition, the CMOS detector performance for the determination of the fraction of slowly diffusing molecules in a primer solution (two-component analysis) is demonstrated. The potential of this new technique for high-throughput confocal-detection-based systems is discussed.

Place, publisher, year, edition, pages
2004. Vol. 9, no 5, 913-921 p.
Keyword [en]
parallel single molecule detection, single-photon detector, complementary metal oxide semiconductor technology, detector arrays, parallel confocal spectroscopy, fluorescence correlation spectroscopy, fluorescence correlation spectroscopy, fan-out element, statistical accuracy, flow, fluctuations
National Category
Electrical Engineering, Electronic Engineering, Information Engineering
URN: urn:nbn:se:kth:diva-23796DOI: 10.1117/1.1781668ISI: 000224356700007ScopusID: 2-s2.0-7044271494OAI: diva2:342495
QC 20100525 QC 20110923Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2011-09-23Bibliographically approved

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