Change search
ReferencesLink to record
Permanent link

Direct link
Pyrosequencing for detection of lamivudine-resistant hepatitis B virus
KTH, Superseded Departments, Biotechnology.
2004 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 42, no 10, 4788-4795 p.Article in journal (Refereed) Published
Abstract [en]

Chronic hepatitis B virus (HBV) infection can cause severe liver disease, including cirrhosis and hepatocellular carcinoma. Lamivudine is a relatively recent alternative to alpha interferon for the treatment of HBV infection, but unfortunately, resistance to lamivudine commonly develops during monotherapy. Lamivudine-resistant HBV mutants display specific mutations in the YMDD (tyrosine, methionine, aspartate, aspartate) motif of the viral polymerase (reverse transcriptase [rt]), which is the catalytic site of the enzyme, i.e., methionine 204 to isoleucine (rtM204I) or valine (rtM204V). The latter mutation is often accompanied by a compensatory leucine-to-methionine change at codon 180 (rtL180M). In the present study, a novel sequencing method, pyrosequencing, was applied to the detection of lamivudine resistance mutations and was compared with direct Sanger sequencing. The new pyrosequencing method had advantages in terms of throughput. Experiments with mixtures of wild-type and resistant viruses indicated that pyrosequencing can detect minor sequence variants in heterogeneous virus populations. The new pyrosequencing method was evaluated with a small number of patient samples, and the results showed that the method could be a useful tool for the detection of lamivudine resistance in the clinical setting.

Place, publisher, year, edition, pages
2004. Vol. 42, no 10, 4788-4795 p.
Keyword [en]
drug-resistance, polymerase, mechanism, mutations, 3tc
National Category
Medical Biotechnology
URN: urn:nbn:se:kth:diva-23805DOI: 10.1128/jcm.42.10.4788-4795.2004ISI: 000224473000055ScopusID: 2-s2.0-5444249657OAI: diva2:342504
QC 20100525 QC 20110922Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2011-09-22Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Odeberg, Jacob
By organisation
In the same journal
Journal of Clinical Microbiology
Medical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 30 hits
ReferencesLink to record
Permanent link

Direct link