Electron beam-induced graft polymerization of acrylic acid and immobilization of arginine-glycine-aspartic acid-containing peptide onto nanopatterned polycaprolactone
2004 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 5, no 6, 2275-2280 p.Article in journal (Refereed) Published
Electron beam- (EB-) induced graft polymerization of acrylic acid and the subsequent immobilization of arginine-glycine-aspartic acid (RGD) peptide onto nanopatterned polycaprolactone with parallel grooves is reported. A high concentration of carboxylic groups was introduced onto the polymer substrate by EB-induced polymerization of acrylic acid. In the coupling of the RGD peptide to the carboxylated polymer surface a three-step peptide immobilization process was used. This process included the activation of surface carboxylic acid into an active ester intermediate by use of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), the introduction of disulfide groups by use of 2-(2-pyridinyldithio)ethanamine tie hydrochloride (PDEA), and final immobilization of the peptide via a thioldisulfide exchange reaction. The extent of coupling was measured by UV spectroscopy. A preliminary study of the in vitro behavior of keratinocytes (NCTC 2544) cultured on the acrylic acid-grafted and RGD peptide-coupled surface showed that most cells grown on the coupled samples had a spread-rounded appearance, while the majority of cells tended to be elongated along the grooves on uncoupled substrates.
Place, publisher, year, edition, pages
2004. Vol. 5, no 6, 2275-2280 p.
high-density polyethylene, cell-adhesion peptides, topographical control, surface modification, different solvents, methacrylic-acid, comb polymers, rgd peptides, in-vitro, wettability
IdentifiersURN: urn:nbn:se:kth:diva-23877DOI: 10.1021/bm049703pISI: 000225091700024ScopusID: 2-s2.0-9744237250OAI: oai:DiVA.org:kth-23877DiVA: diva2:342576
QC 20100525 QC 201109152010-08-102010-08-102011-09-15Bibliographically approved