7-deaza-2 '-deoxyadenosine-5 '-triphosphate as an alternative nucleotide for the pyrosequencing technology
2004 (English)In: Nucleosides, Nucleotides & Nucleic Acids, ISSN 1525-7770, E-ISSN 1532-2335, Vol. 23, no 10, 1583-1594 p.Article in journal (Refereed) Published
A new adenosine nucleotide analog suitable for the Pyrosequencing method is presented. The new analog, 7-deaza-2'-deoxyadenosine-5'-triphosphate (c(7)dATP), has virtually the. same low substrate specificity for luciferase as the currently used analog, 2'-deoxyadenosine-5'-O-(1-thiotriphosphate) (dATPalphaS). The inhibitory effect dATPalphaS displays on the nucleotide degrading activity of apyrase was reduced significantly by substituting the c(7)dATP for the dATPalphaS. Both analogs show high stability after long time storage at +8degreesC. Furthermore, with the new nucleotide a read length of up to 100 bases was obtained for several templates from fungi, bacteria and viruses.
Place, publisher, year, edition, pages
2004. Vol. 23, no 10, 1583-1594 p.
DNA sequencing, pyrosequencing technology, 7-deaza-2 '-deoxyadenosine-5 '-triphosphate, 2 '-deoxyadenosine-5 '-O-(1-thiotriphosphate), luciferase, firefly luciferase, escherichia-coli, dna-polymerase, pyrophosphate
IdentifiersURN: urn:nbn:se:kth:diva-23938DOI: 10.1081/ncn-20031402ISI: 000225549600002ScopusID: 2-s2.0-9644272463OAI: oai:DiVA.org:kth-23938DiVA: diva2:342637
QC 20100525 QC 201111012010-08-102010-08-102012-03-22Bibliographically approved