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Biotechnological applications for surface-engineered bacteria
KTH, Superseded Departments, Biotechnology.
KTH, Superseded Departments, Biotechnology.ORCID iD: 0000-0002-9282-0174
2004 (English)In: Biotechnology and applied biochemistry, ISSN 0885-4513, E-ISSN 1470-8744, Vol. 40, 209-228 p.Article, review/survey (Refereed) Published
Abstract [en]

Display of heterologous proteins on the surface of micro-organisms, enabled by means of recombinant DNA technology, has become an increasingly popular strategy in microbiology, biotechnology and vaccinology. Both Gram-negative and Gram-positive bacteria have been investigated for potential applications. The present review will describe the most commonly used systems for bacterial display, with a focus on the biotechnology applications. Live bacterial vaccine-delivery vehicles have long been investigated through the surface display of foreign antigens and, recently, 'second-generation' vaccine-delivery vehicles have been generated by the addition of mucosal targeting signals, as a means to increase immune responses. Engineered bacteria have also the potential to act as novel microbial biocatalysts with heterologous enzymes immobilized as surface exposed on the bacterial cell surface. They provide the potential for new types of whole-cell diagnostic devices, since single-chain antibodies and other type of tailor-made binding proteins can be displayed on bacteria. Bacteria with increased binding capacity for certain metal ions can be created, and potential environmental or biosensor applications for such recombinant bacteria as biosorbents are being explored. Certain bacteria have also been employed to display various polypeptide libraries for use as devices in in vitro selection applications. Part of the present review has been devoted to a more in-depth description of a promising Gram-positive display system, i.e. Staphylococcus carnosus, and its applications. The review describes the basic principles of the different bacterial display systems and discusses current uses and possible future trends of these emerging technologies.

Place, publisher, year, edition, pages
2004. Vol. 40, 209-228 p.
Keyword [en]
fluorescence-activated cell sorting (FACS), gram-positive bacteria, surface-engineered bacteria, vaccine delivery, cellulose-binding domain, ice-nucleation protein, coli cell-surface, toxin-b-subunit, outer-membrane proteins, foreign antigenic determinants, organophosphate nerve agents, random peptide libraries, gram-positive bacteria, lyme-disease vaccine
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-23946DOI: 10.1042/BA20040014ISI: 000225622100001ScopusID: 2-s2.0-10444241949OAI: diva2:342645
QC 20100525 QC 20110913Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2011-09-23Bibliographically approved

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