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The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems
KTH, Superseded Departments, Biotechnology.
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2004 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 25, no 21-22, 3740-3745 p.Article in journal (Refereed) Published
Abstract [en]

A novel method for studying unlabeled living mammalian cells based on their autofluorescence (AF) signal in a prototype microfluidic device is presented. When combined, cellular AF detection and microfluidic devices have the potential to facilitate high-throughput analysis of different cell populations. To demonstrate this, unlabeled cultured cells in microfluidic devices were excited with a 488 nm excitation light and the AF emission (> 505 nm) was detected using a confocal fluorescence microscope (CFM). For example, a simple microfluidic three-port glass microstructure was used together with conventional electroosmotic flow (EOF) to switch the direction of the fluid flow. As a means to test the potential of AF-based cell sorting in this microfluidic device, granulocytes were successfully differentiated from human red blood cells (RBCs) based on differences in AF This study demonstrated the use of a simple microfabricated device to perform high-throughput live cell detection and differentiation without the need for cell-specific fluorescent labeling dyes and thereby reducing the sample preparation time. Hence, the combined use of microfluidic devices and cell AF may have many applications in single-cell analysis.

Place, publisher, year, edition, pages
2004. Vol. 25, no 21-22, 3740-3745 p.
Keyword [en]
autofluorescence, microfluidic, miniaturization, red blood cell, single-cell analysis, spectroscopy, transport
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-23952DOI: 10.1002/elps.200406070ISI: 000225679000024Scopus ID: 2-s2.0-10944267669OAI: oai:DiVA.org:kth-23952DiVA: diva2:342651
Note
QC 20100525 QC 20110915Available from: 2010-08-10 Created: 2010-08-10 Last updated: 2011-09-15Bibliographically approved

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