An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy
2004 (English)In: Journal of Physics D: Applied Physics, ISSN 0022-3727, E-ISSN 1361-6463, Vol. 37, no 23, 3296-3303 p.Article in journal (Refereed) Published
Fluorescence imaging is used widely in microscopy and macroscopic imaging applications for fields ranging from biomedicine to materials science. A critical component for any fluorescence imaging system is the excitation source. Traditionally, wide-field systems use filtered thermal or arc-generated white light sources, while point scanning confocal microscope systems require spatially coherent (point-like) laser sources. Unfortunately, the limited range of visible wavelengths available from conventional laser sources constrains the design and usefulness of fluorescent probes in confocal microscopy. A 'hands-off' laser-like source, electronically tunable across the visible spectrum, would be invaluable for fluorescence imaging and provide new opportunities, e.g. automated excitation fingerprinting and in situ measurement of excitation cross-sections. Yet more information can be obtained using fluorescence lifetime imaging (FLIM), which requires that the light source be pulsed or rapidly modulated. We show how a white light continuum, generated by injecting femtosecond optical radiation into a micro-structured optical fibre, coupled with a simple prism-based tunable filter arrangement, can fulfil all these roles as a continuously electronically tunable (435-1150 nm) visible ultrafast light source in confocal, wide-field and FLIM systems.
Place, publisher, year, edition, pages
2004. Vol. 37, no 23, 3296-3303 p.
photonic crystal fibers, supercontinuum generation, anomalous-dispersion, continuum generation, spatial-resolution, raman-scattering, optical-fiber, multiphoton, cell, pulses
IdentifiersURN: urn:nbn:se:kth:diva-23971DOI: 10.1088/0022-3727/37/23/011ISI: 000225915200014OAI: oai:DiVA.org:kth-23971DiVA: diva2:342670
QC 201005252010-08-102010-08-10Bibliographically approved