Variability of a glucose phosphotransferase system permease in Mycoplasma mycoides subsp mycoides Small Colony
2004 (English)In: Microbiology, ISSN 1350-0872, E-ISSN 1465-2080, Vol. 150, 4009-4022 p.Article in journal (Refereed) Published
Intraclonal antigenic variation in pathogenic mycoplasma species is considered an important feature of host-pathogen interaction. Such intraclonal protein variation was observed for the interaction of Mycoplasma mycoides subsp. mycoides Small Colony, the agent of contagious bovine pleuropneumonia, with mAb 3F3. Colony immunostaining allows the definition of 3F3 ON- and 3F3 OFF-type variants, which revert at low frequency. Targets of mAb 3F3 were shown to be surface located, and resided on multiple polypeptides in the 58-68 kDa size range. Phage display and a genomic database were combined to determine the gene encoding the proteins recognized by mAb 3F3. A gene encoding the putative permease of the glucose phosphotransferase system was identified. Genome sequence analysis of strain PG1 revealed two highly similar copies of this gene, resulting from duplication of the chromosomal region carrying the gene. Southern blot analysis demonstrated the presence of this duplication in almost every African strain tested, but not in European strains. DNA analysis revealed that ON/OFF switching is governed by a base substitution occurring upstream of the coding region for the 3F3 epitope. This event generates a stop codon that results in the premature termination of the PtsG protein.
Place, publisher, year, edition, pages
2004. Vol. 150, 4009-4022 p.
contagious bovine pleuropneumonia, variable surface lipoproteins, antigenic variation, phase variation, dna inversions, high-frequency, phage-display, gene family, molecular characterization, frameshift mutation
IdentifiersURN: urn:nbn:se:kth:diva-23980DOI: 10.1099/mic.0.27247-0ISI: 000226043000014ScopusID: 2-s2.0-11044237741OAI: oai:DiVA.org:kth-23980DiVA: diva2:342679
QC 20100525 QC 201109232010-08-102010-08-102011-09-23Bibliographically approved