Chromosomal sequencing using a PCR-based biotin-capture method allowed isolation of the complete gene for the outer membrane protein A of Klebsiella pneumoniae
1998 (English)In: Gene, ISSN 0378-1119, E-ISSN 1879-0038, Vol. 210, no 1, 93-101 p.Article in journal (Refereed) Published
By employing a novel biotin-and PCR-assisted capture method, which allows determination of unknown sequences on chromosomal DNA. the gene for the outer membrane protein A (OmpA) of Klebsiella pneumoniae has been isolated and sequenced to completion. The method involves linear amplification of DNA from a biotinylated primer annealing to a region with known sequence. After capture of the amplified single-stranded DNA on to paramagnetic beads, unspecifically annealing primers, i.e. arbitrary primers, were used to generate fragments with only partly determined nt sequences. The homology of the sequenced gene to ompAs of related bacteria is discussed. The ompA gene was assembled for intracellular expression in Escherichia coli, and two different fusion proteins were produced and recovered with good yields. The importance of the novel chromosomal sequencing method for gene isolation in general and the potential use of the OmpA fusion proteins are discussed. (C) 1998 Elsevier Science B.V.
Place, publisher, year, edition, pages
1998. Vol. 210, no 1, 93-101 p.
solid-phase technology, biotin-assisted capture, outer membrane protein A, Klebsiella pneumoniae, fusion protein
IdentifiersURN: urn:nbn:se:kth:diva-24089ISI: 000073075300012OAI: oai:DiVA.org:kth-24089DiVA: diva2:343305
QC 201008132010-08-132010-08-132010-08-13Bibliographically approved