Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Gene discovery in the wood-forming tissues of poplar: Analysis of 5,692 expressed sequence tags
Show others and affiliations
1998 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 95, no 22, 13330-13335 p.Article in journal (Refereed) Published
Abstract [en]

A rapidly growing area of genome research is the generation of expressed sequence tags (ESTs) in which large numbers of randomly selected cDNA clones are partially sequenced. The collection of ESTs reflects the level and complexity of gene expression in the sampled tissue, To date, the majority of plant ESTs are from nonwoody plants such as Arabidopsis, Brassica, maize, and rice. Here, we present a large-scale production of ESTs from the wood-forming tissues of two poplars, Populus tremula L, x tremuloides Michx, and Populus trichocarpa 'Trichobel.' The 5,692 ESTs analyzed represented a total of 3,719 unique transcripts for the two cDNA libraries, Putative functions could be assigned to 2,245 of these transcripts that corresponded to 820 protein functions. Of specific interest to forest biotechnology are the 4% of ESTs involved in various processes of cell wall formation, such as lignin and cellulose synthesis, 5% similar to developmental regulators and members of known signal transduction pathways, and 2% involved in hormone biosynthesis. An additional 12% of the ESTs show ed no significant similarity to any other DNA or protein sequences in existing databases. The absence of these sequences from public databases may indicate a specific role for these proteins in wood formation. The cDNA libraries and the accompanying database are valuable resources for forest research directed toward understanding the genetic control of wood formation and future endeavors to modify wood and fiber properties for industrial use.

Place, publisher, year, edition, pages
1998. Vol. 95, no 22, 13330-13335 p.
Keyword [en]
cambium, forestry, functional genomics, xylem, xylogenesis
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-24087ISI: 000076757300110OAI: oai:DiVA.org:kth-24087DiVA: diva2:343327
Note
QC 20100813Available from: 2010-08-13 Created: 2010-08-13 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Sequencing of genes and genomes
Open this publication in new window or tab >>Sequencing of genes and genomes
1998 (English)Doctoral thesis, comprehensive summary (Other scientific)
Place, publisher, year, edition, pages
Stockholm: KTH, 1998. 63 p.
Keyword
DNA sequencing, genome analysis, expressed sequence tag, expression analysis, complementary DNA, biotin-capture, solid-phase technology, automation, poplar
National Category
Industrial Biotechnology
Identifiers
urn:nbn:se:kth:diva-2714 (URN)91-7170-315-2 (ISBN)
Public defence
1998-11-01, 00:00
Note
QC 20100813Available from: 2000-01-01 Created: 2000-01-01 Last updated: 2010-08-13Bibliographically approved

Open Access in DiVA

No full text

Authority records BETA

Sterky, FredrikHolmberg, AndersUhlen, Mathias

Search in DiVA

By author/editor
Sterky, FredrikHolmberg, AndersMohammad Amini, Amini BahramUhlen, MathiasLundeberg, Joakim
By organisation
Biotechnology
In the same journal
Proceedings of the National Academy of Sciences of the United States of America
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 87 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf