Change search
ReferencesLink to record
Permanent link

Direct link
Chitosan-N-poly (ethylene oxide) brush polymers for reduced nonspecific protein adsorption
Show others and affiliations
2007 (English)In: Journal of Colloid and Interface Science, ISSN 0021-9797, E-ISSN 1095-7103, Vol. 305, no 1, 62-71 p.Article in journal (Refereed) Published
Abstract [en]

The possibility of using a novel comb polymer consisting of a chitosan backbone with grafted 44 units long poly(ethylene oxide) side chains for reducing nonspecific protein adsorption to gold surfaces functionalized by COOH-terminated thiols has been explored. The comb polymer was attached to the surface in three different ways: by solution adsorption, covalent coupling, and microcontact printing. The protein repellant properties were tested by monitoring the adsorption of bovine serum albumin and fibrinogen employing surface plasmon resonance and imaging null ellipsometry. It was found that a significant reduction in protein adsorption is achieved as the comb polymer layer is sufficiently dense. For solution adsorption this was achieved by adsorption from high pH solutions. On the other hand, the best performance of the microcontact printed surfaces was obtained when the stamp was inked either at low or at high pH. For a given comb polymer layer thickness/poly(ethylene oxide) density, significant differences in protein repellant properties were observed between the different preparation methods, and it is suggested that a reduction in the mobility of the comb polymer layer generated by covalent attachment favors a reduced protein adsorption. (c) 2006 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2007. Vol. 305, no 1, 62-71 p.
Keyword [en]
comb polymer, comb polyelectrolyte, chitosan, poly(ethylene oxides), nonspecific binding, adsorption, microcontact printing, surface plasmon, resonance, imaging null ellipsometry
National Category
Chemical Sciences
URN: urn:nbn:se:kth:diva-24348DOI: 10.1016/j.jcis.2006.09.058ISI: 000243121600009ScopusID: 2-s2.0-33750939636OAI: diva2:347432
QC 20100903Available from: 2010-09-03 Created: 2010-09-03 Last updated: 2010-09-03Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Dedinaite, AndraClaesson, Per Martin
By organisation
Surface Chemistry
In the same journal
Journal of Colloid and Interface Science
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 21 hits
ReferencesLink to record
Permanent link

Direct link