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Lignin composition in cambial tissues of poplar
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology.
2006 (English)In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 44, no 11-12, 700-706 p.Article in journal (Refereed) Published
Abstract [en]

The cambial tissues of a Populus balsamifera, Balsam poplar clone were studied during a growth season. The Klason and acid-soluble lignin contents were determined as well as the carbohydrate monomer distribution and the protein content. Both the phloem and the xylem sides of the cambial region were examined. The samples were analyzed by thioacidolysis and structures of dimeric products were determined by mass spectrometry after desulphuration. Chemical analysis of samples during the growth season was combined with microscopy of embedded specimens that showed the state of cell differentiation at the time of sampling. In spring and early summer, growth is very rapid and the intention was to collect tissue in which exclusively the middle lamella/primary cell wall had begun to lignify. The Klason lignin, protein content and carbohydrate monomer distribution showed that all the specimens from the cambial tissues sampled during a growth season contained predominantly middle lamella and primary walls; except for the developing xylem sampled in August where the carbohydrate composition showed that secondary walls were present. Thioacidolysis showed that the lignin from the cambial tissues had more condensed structures than the lignin from the reference balsam poplar clone wood. More guaiacyl than syringyl units were detected and mass spectrometry showed that the cambial tissues contained more lignin structures with end-groups than the reference sample. These results suggest that lignification in the cambial layer and early developing xylem may take place predominantly in a bulk fashion during the summer. (c) 2006 Published by Elsevier Masson SAS.

Place, publisher, year, edition, pages
2006. Vol. 44, no 11-12, 700-706 p.
Keyword [en]
lignin, thioacidolysis, cambial tissues, middle lamella, Populus balsamifera, balsam poplar, developing xylem, developing phloem
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-24605DOI: 10.1016/j.plaphy.2006.10.012ISI: 000243445700010Scopus ID: 2-s2.0-33845504447OAI: oai:DiVA.org:kth-24605DiVA: diva2:352228
Note

QC 20100920

Available from: 2010-09-20 Created: 2010-09-20 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Composition of lignin in outer cell-wall layers
Open this publication in new window or tab >>Composition of lignin in outer cell-wall layers
2006 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

The composition of lignin in the outer cell-wall layers of spruce and poplar has been studied and the data obtained have been compared with those of the mature reference wood in which the secondary cell wall predominates. Materials with exclusively or predominantly outer cell-wall layers were examined. Accurate data relating to the lignin monomer composition and the number of β-O-4´ bonds were obtained from pure middle lamella/primary cell wall lignin. Firstly, a 10 000 year old white spruce material, with most of the secondary cell wall missing, was studied. The aged lignin was composed of guaiacyl units only, and was slightly more condensed but otherwise similar to the reference lignin.

Secondly, the developing xylem of a Norway spruce clone was analyzed during a growth season. In spring and early summer, growth is very rapid and the intention was to sample tissues in which the secondary cell-wall layers had not yet lignified, but where the outer layers at least had started to lignify. Microscopy, Klason lignin and carbohydrate analyses showed that the lignin in the developing xylem of samples from mid-June was located exclusively in the middle lamella. The lignin was more condensed, was composed of guaiacyl units only and contained more end-groups than the reference Norway spruce wood.

Thirdly, the cambial tissues of a Balsam poplar clone were surveyed during a growth season. Both the phloem side and the xylem side of the cambial region were examined. The Klason lignin content and carbohydrate monomer distribution showed that in June and August the tissues on the phloem side contained material with mainly middle lamella/primary walls. In June, the xylem side in the cambial region contained mainly middle lamella/primary walls, and in August the secondary cell wall carbohydrates were being deposited. Both tissues contained lignin that was more condensed and had more end-groups than the reference lignin. In mid-June, the developing xylem had a ratio of syringyl to guaiacyl units of 0.6, whereas the ratio for the reference wood was 1.3.

In the final study, lignin from the primary cell walls from a hybrid aspen cell suspension culture was investigated. The lignin contained only guaiacyl units which were more condensed than those observed in the reference poplar wood.

Place, publisher, year, edition, pages
Stockholm: KTH, 2006. 53 p.
Series
TRITA-FPT-Report, ISSN 1652-2443 ; 2006:16
Keyword
Lignin, thioacidolysis, primary wall, middle lamella, Populus balsamifera, Populus tremula L. x P. tremuloides Michx., Picea abies, Picea glauca
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:kth:diva-4036 (URN)
Public defence
2006-06-15, Sal F3, Lindstedtsvägen 26, Stockholm, 14:00
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Supervisors
Note

QC 20100920

Available from: 2006-06-02 Created: 2006-06-02 Last updated: 2016-12-21Bibliographically approved

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