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Lignin isolated from primary walls of hybrid aspen cell cultures indicates significant differences in lignin structure between primary and secondary cell wall
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology.
KTH, School of Biotechnology (BIO), Biochemistry.ORCID iD: 0000-0002-5368-4296
KTH, School of Biotechnology (BIO), Biochemistry.
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology.
2005 (English)In: Plant physiology and biochemistry (Paris), ISSN 0981-9428, E-ISSN 1873-2690, Vol. 43, no 8, 777-785 p.Article in journal (Refereed) Published
Abstract [en]

Hybrid aspen (Populus tremula x tremuloides) cell cultures were grown for 7, 14 and 21 days. The cell cultures formed primary cell walls but no secondary cell wall according to carbohydrate analysis and microscopic characterization. The primary walls were lignified, increasingly with age, according to Klason lignin analysis. Presence of lignin in the primary walls, with a higher content in 21-day old cells than in 7-day old cells, was further Supported by phloroglucinol/HCI reagent test and confocal microscopy after both immunolocalization and staining with acriflavin. Both laccase and peroxidase activity were found in the cultures and the activity increased during lignin formation. The lignin from the cell culture material was compared to lignin from mature aspen wood, where most of the lignin originates in the secondary cell wall, and which served as our secondary cell wall control. Lignin from the cell walls was isolated and characterized by thioacidolysis followed by gas chromatography and mass spectrometry. The lignin in the cell cultures differed from lignin of mature aspen wood in that it consisted exclusively of guaiacyl units, and had a more condensed structure. Five lignin structures were identified by mass spectrometry in the cell suspension cultures. The results indicate that the hybrid aspen cell culture used in this investigation may be a convenient experimental system for studies of primary cell wall lignin.

Place, publisher, year, edition, pages
2005. Vol. 43, no 8, 777-785 p.
Keyword [en]
primary cell wall lignin hybrid aspen cell culture
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-24606DOI: 10.1016/j.plaphy.2005.07.007ISI: 000233144100006Scopus ID: 2-s2.0-26844492086OAI: oai:DiVA.org:kth-24606DiVA: diva2:352233
Note
QC 20100920Available from: 2010-09-20 Created: 2010-09-20 Last updated: 2017-12-12Bibliographically approved
In thesis
1. Composition of lignin in outer cell-wall layers
Open this publication in new window or tab >>Composition of lignin in outer cell-wall layers
2006 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

The composition of lignin in the outer cell-wall layers of spruce and poplar has been studied and the data obtained have been compared with those of the mature reference wood in which the secondary cell wall predominates. Materials with exclusively or predominantly outer cell-wall layers were examined. Accurate data relating to the lignin monomer composition and the number of β-O-4´ bonds were obtained from pure middle lamella/primary cell wall lignin. Firstly, a 10 000 year old white spruce material, with most of the secondary cell wall missing, was studied. The aged lignin was composed of guaiacyl units only, and was slightly more condensed but otherwise similar to the reference lignin.

Secondly, the developing xylem of a Norway spruce clone was analyzed during a growth season. In spring and early summer, growth is very rapid and the intention was to sample tissues in which the secondary cell-wall layers had not yet lignified, but where the outer layers at least had started to lignify. Microscopy, Klason lignin and carbohydrate analyses showed that the lignin in the developing xylem of samples from mid-June was located exclusively in the middle lamella. The lignin was more condensed, was composed of guaiacyl units only and contained more end-groups than the reference Norway spruce wood.

Thirdly, the cambial tissues of a Balsam poplar clone were surveyed during a growth season. Both the phloem side and the xylem side of the cambial region were examined. The Klason lignin content and carbohydrate monomer distribution showed that in June and August the tissues on the phloem side contained material with mainly middle lamella/primary walls. In June, the xylem side in the cambial region contained mainly middle lamella/primary walls, and in August the secondary cell wall carbohydrates were being deposited. Both tissues contained lignin that was more condensed and had more end-groups than the reference lignin. In mid-June, the developing xylem had a ratio of syringyl to guaiacyl units of 0.6, whereas the ratio for the reference wood was 1.3.

In the final study, lignin from the primary cell walls from a hybrid aspen cell suspension culture was investigated. The lignin contained only guaiacyl units which were more condensed than those observed in the reference poplar wood.

Place, publisher, year, edition, pages
Stockholm: KTH, 2006. 53 p.
Series
TRITA-FPT-Report, ISSN 1652-2443 ; 2006:16
Keyword
Lignin, thioacidolysis, primary wall, middle lamella, Populus balsamifera, Populus tremula L. x P. tremuloides Michx., Picea abies, Picea glauca
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:kth:diva-4036 (URN)
Public defence
2006-06-15, Sal F3, Lindstedtsvägen 26, Stockholm, 14:00
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Supervisors
Note

QC 20100920

Available from: 2006-06-02 Created: 2006-06-02 Last updated: 2016-12-21Bibliographically approved

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