Single particle refinement in electron crystallography: A pilot study
2007 (English)In: Journal of Structural Biology, ISSN 1047-8477, E-ISSN 1095-8657, Vol. 160, no 3, 344-352 p.Article in journal (Refereed) Published
Electron crystallography can be used to determine the structures of membrane proteins at near-atomic resolution in some cases. However, most electron crystallography projects remain at a resolution around 10 angstrom. This might be partly due to lack of flatness of many two-dimensional crystals. We have investigated this problem and suggest single particle processing of locally averaged unit cells to improve the quality and possibly the resolution of three-dimensional maps. Applying this method to the secondary transporter melibiose permease we have calculated a three-dimensional map that is clearer and easier to interpret than the map derived using purely electron-crystallographic methods.
Place, publisher, year, edition, pages
2007. Vol. 160, no 3, 344-352 p.
electron crystallography, single particle reconstruction, membrane proteins, crystal distortion
IdentifiersURN: urn:nbn:se:kth:diva-24889DOI: 10.1016/j.jsb.2007.09.001ISI: 000251473500010OAI: oai:DiVA.org:kth-24889DiVA: diva2:353879
QC 201009292010-09-292010-09-292013-07-19Bibliographically approved