Cultivation of COS7-cells using extracellular matrix in 3D microfluidic surface enlarged structure
2005 (English)In: Micro Total Analysis Systems 2004, 2005, no 297, 261-263 p.Conference paper (Refereed)
This paper presents a novel method to cultivate cells in a controlled 3D surface enlarged micro-environment. The 3D environment is achieved by insertion of a gel-like extracellular matrix (ECM) mixed with cells into a micromachined silicon fluid structure. Shrinking of the gel enables further flow through the channel. Due to the structure design the shrinking is non-uniform, which results in an increased surface area. With the proposed design cells are alive and viable after 72 h of incubation within the chip.
Place, publisher, year, edition, pages
2005. no 297, 261-263 p.
, ROYAL SOCIETY OF CHEMISTRY SPECIAL PUBLICATIONS, ISSN 0260-6291
3D-cultivation, matrigel, ECM, microfluidic structure
IdentifiersURN: urn:nbn:se:kth:diva-24781ISI: 000229017400086ISBN: 0-85404-896-0OAI: oai:DiVA.org:kth-24781DiVA: diva2:355272
8th International Conference on Miniaturized Systems for Chemistry and Life Sciences Malmo, SWEDEN, SEP 26-30, 2004
QC 201010062010-10-062010-09-272010-10-06Bibliographically approved