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Cultivation of COS7-cells using extracellular matrix in 3D microfluidic surface enlarged structure
KTH, School of Electrical Engineering (EES), Microsystem Technology.ORCID iD: 0000-0002-3996-9279
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.
KTH, School of Electrical Engineering (EES), Microsystem Technology.
KTH, School of Engineering Sciences (SCI), Applied Physics, Cell Physics.ORCID iD: 0000-0003-0578-4003
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2005 (English)In: Micro Total Analysis Systems 2004, 2005, no 297, 261-263 p.Conference paper, Published paper (Refereed)
Abstract [en]

This paper presents a novel method to cultivate cells in a controlled 3D surface enlarged micro-environment. The 3D environment is achieved by insertion of a gel-like extracellular matrix (ECM) mixed with cells into a micromachined silicon fluid structure. Shrinking of the gel enables further flow through the channel. Due to the structure design the shrinking is non-uniform, which results in an increased surface area. With the proposed design cells are alive and viable after 72 h of incubation within the chip.

Place, publisher, year, edition, pages
2005. no 297, 261-263 p.
Series
ROYAL SOCIETY OF CHEMISTRY SPECIAL PUBLICATIONS, ISSN 0260-6291
Keyword [en]
3D-cultivation, matrigel, ECM, microfluidic structure
Identifiers
URN: urn:nbn:se:kth:diva-24781ISI: 000229017400086ISBN: 0-85404-896-0 (print)OAI: oai:DiVA.org:kth-24781DiVA: diva2:355272
Conference
8th International Conference on Miniaturized Systems for Chemistry and Life Sciences Malmo, SWEDEN, SEP 26-30, 2004
Note
QC 20101006Available from: 2010-10-06 Created: 2010-09-27 Last updated: 2010-10-06Bibliographically approved

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Brismar, HjalmarStemme, Göran

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