Genotyping by dynamic heating of monolayered beads on a microheater surface
2005 (English)In: Micro Total Analysis Systems 2004 / [ed] Laurell T; Nilsson J; Jensen K; Harrison DJ, Royal Society of Chemistry, 2005, no 297, 303-305 p.Conference paper (Refereed)
This paper presents SNP scoring by DASH technology by employing dynamic heating of beads immobilized on a chip with integrated heater and sensor. The microfabricated chip designed for open-surface DNA analysis allows fast, well controllable temperature ramping and homogeneous temperature distribution over the entire heater area. Beads containing DNA duplexes are immobilized on the surface of the chip by microcontact printing using a PDMS stamp. All three possible variants of a SNP site of an oligonucleotide were accurately scored using the bead-based DASH approach. Using the chip, the total analysis time could easily be reduced by a factor 2 compared with the current DASH assay.
Place, publisher, year, edition, pages
Royal Society of Chemistry, 2005. no 297, 303-305 p.
, Royal Society of Chemistry Special Publications, ISSN 0260-6291
DASH, microheater, microcontact printing, microspheres, SNP
Other Electrical Engineering, Electronic Engineering, Information Engineering
IdentifiersURN: urn:nbn:se:kth:diva-24782ISI: 000229017400100ISBN: 0-85404-896-0OAI: oai:DiVA.org:kth-24782DiVA: diva2:355305
8th International Conference on Miniaturized Systems for Chemistry and Life Sciences Malmo, Sweden, SEP 26-30, 2004
QC 201010062010-10-062010-09-272016-07-22Bibliographically approved