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Genotyping by dynamic heating of monolayered beads on a microheater surface
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).ORCID iD: 0000-0002-9327-2544
KTH, School of Electrical Engineering (EES), Microsystem Technology (Changed name 20121201).
Karolinska Institute.
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2005 (English)In: Micro Total Analysis Systems 2004 / [ed] Laurell T; Nilsson J; Jensen K; Harrison DJ, Royal Society of Chemistry, 2005, no 297, 303-305 p.Conference paper, Published paper (Refereed)
Abstract [en]

This paper presents SNP scoring by DASH technology by employing dynamic heating of beads immobilized on a chip with integrated heater and sensor. The microfabricated chip designed for open-surface DNA analysis allows fast, well controllable temperature ramping and homogeneous temperature distribution over the entire heater area. Beads containing DNA duplexes are immobilized on the surface of the chip by microcontact printing using a PDMS stamp. All three possible variants of a SNP site of an oligonucleotide were accurately scored using the bead-based DASH approach. Using the chip, the total analysis time could easily be reduced by a factor 2 compared with the current DASH assay.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2005. no 297, 303-305 p.
Series
Royal Society of Chemistry Special Publications, ISSN 0260-6291
Keyword [en]
DASH, microheater, microcontact printing, microspheres, SNP
National Category
Other Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
URN: urn:nbn:se:kth:diva-24782ISI: 000229017400100ISBN: 0-85404-896-0 (print)OAI: oai:DiVA.org:kth-24782DiVA: diva2:355305
Conference
8th International Conference on Miniaturized Systems for Chemistry and Life Sciences Malmo, Sweden, SEP 26-30, 2004
Note

QC 20101006

Available from: 2010-10-06 Created: 2010-09-27 Last updated: 2016-07-22Bibliographically approved

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Authority records BETA

Haasl, SjoerdStemme, Göran

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CiteExportLink to record
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  • apa
  • harvard1
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Output format
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