Bacterial-growth inhibiting properties of multilayers formed with modified polyvinylamine
2011 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 88, no 1, 115-120 p.Article in journal (Refereed) Published
New methods are needed to fight antibiotic-resistant bacteria. One alternative that has been proposed is non-leaching, permanently antibacterial surfaces. In this study, we test multilayers formed with antibacterial cationic polyvinylamine (PVAm) and polyacrylic acid (PAA) in a growth-inhibition assay. Both hydrophobically modified and native PVAm were investigated. Multilayers did reduce the bacterial growth, as compared to single layers. However, the sampling time in the assay was critical, as the treated surface area is a capacity-limiting factor. After 2 h incubation, a maximal growth inhibition of more than 99% was achieved with multilayers. In contrast, after 8 h we observed a maximal growth-inhibition of 40%. At longer incubation times, the surface becomes saturated, which explains the observed time-dependent effectiveness. The polymers giving multilayers with the strongest growth-inhibiting properties were native PVAm and PVAm modified with C(8), which also were the polymers with highest charge density. We therefore conclude that this effect is mainly an electrostatically driven process. Viability staining using a fluorescent stain showed a high viability rate of the adhered bacteria. The multilayers are therefore more bacteriostatic than antibacterial.
Place, publisher, year, edition, pages
2011. Vol. 88, no 1, 115-120 p.
Polyvinylamine, Hydrophobic modification, Polyacrylic acid, Polyelectrolyte multilayers, Antibacterial surfaces
IdentifiersURN: urn:nbn:se:kth:diva-25252DOI: 10.1016/j.colsurfb.2011.06.023ISI: 000295344800015ScopusID: 2-s2.0-80052083682OAI: oai:DiVA.org:kth-25252DiVA: diva2:356829
QC 20101014 Updated from submitted to published.2010-10-142010-10-142012-02-28Bibliographically approved