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Determination of Dissociation Constants on Quartz Crystal Microbalance Surfaces by Polymer Signal Enhancement
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
(English)Manuscript (preprint) (Other academic)
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-25284OAI: oai:DiVA.org:kth-25284DiVA: diva2:357072
Note

QC 20101015

Available from: 2010-10-15 Created: 2010-10-15 Last updated: 2016-06-08Bibliographically approved
In thesis
1. Signal Enhancement by Dynamic Polymers in Quartz Crystal Microbalance Applications
Open this publication in new window or tab >>Signal Enhancement by Dynamic Polymers in Quartz Crystal Microbalance Applications
2009 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

 This licentiate thesis presents studies of signal enhancement by dynamic polymers in quartz crystal microbalance (QCM) applications. The aim of the study has been to determine the potential for the use of polymers on QCM sensors for signal enhancement in molecular interaction studies. A method for synthesis of polymer sensor surfaces on QCM substrates was developed based on an iniferter photo polymerization technique. Polymerized poly(acrylic acid) and poly(acryl amide) surfaces were extensively characterized with electron spectroscopy for chemical analysis (ESCA), infrared reflection absorption spectroscopy (IRAS) and atomic force microscopy (AFM). The QCM response dynamics of these surfaces to pH changes was studied and the carboxyl containing surfaces exhibited large, reversible and highly reproducible frequency shifts due to expansion of deprotonated polymer chains. Surface acid dissociation constants (pKa) were determined for poly(acrylic acid) surfaces, carboxymethyldextran surfaces and self-assembled monolayer carboxyl surfaces by means of pH titration in QCM flow-through instrumentation. Surface pKa data was consistent with available literature data. The sensitivity enhancement for molecular interaction studies was investigated for poly(acrylic acid) coated QCM sensors with a model system consisting of immobilized biotin and an anti-biotin Fab fragment. Binding responses of antibiotin Fab to immobilized biotin was revealed to be 11 times higher than the corresponding results for a carboxylated SAM surface.

 

Place, publisher, year, edition, pages
Stockholm: KTH, 2009. 41 p.
Series
Trita-CHE-Report, ISSN 1654-1081 ; 2009:17
Keyword
Signal enhancement, QCM, polyelectrolytes, hydrogel, iniferter, photopolymerization, dynamic polymers
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-10305 (URN)978-91-7415-307-1 (ISBN)
Presentation
2009-05-27, E3, Osquars backe 14, Stockholm, 10:00 (English)
Opponent
Supervisors
Available from: 2009-05-07 Created: 2009-05-06 Last updated: 2010-10-15Bibliographically approved

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