The correlation between antigen solubility and immunogenicity
(English)Manuscript (preprint) (Other academic)
In antigen design, several characteristics contribute to the final success of the antigen to elicit the desired immunogenicity. However, it is difficult to screen theses attributes due to the variability within the host immune receptor repertoire. Herein, with the massive numeral of immunizations performed within a proteome-wide endeavor to produce affinity reagents to human proteins, the correlation between the solubility of the antigens and the immunogenicity was investigated. We showed that increased solubility of the antigen resulted in higher success rate in provoking the immune defense as well as higher antibody titers. We have also shown, that the increased antibody titers after affinity purifications indeed reflectthe concentration of target specific antibodies within the serum. Finally, the amino acid composition of soluble antigens was determined to be over-represented in polar residues.
Other Industrial Biotechnology
IdentifiersURN: urn:nbn:se:kth:diva-25854OAI: oai:DiVA.org:kth-25854DiVA: diva2:360245
QC 201011022010-11-022010-11-022010-11-02Bibliographically approved