Surrogate antigens as targets for proteome-wide binder selection
2011 (English)In: New Biotechnology, ISSN 1871-6784, Vol. 28, no 4, 302-311 p.Article in journal (Refereed) Published
In the last decade, many initiatives have been taken to develop antibodies for proteome-wide studies, as well as characterization and validation of clinically relevant disease biomarkers. Phage display offers many advantages compared to conventional antibody generation by immunization and hybridoma technology, since it is an unlimited resource of affinity reagents without batch-to-batch variation and is amendable for high throughput. One of the major bottlenecks to proteome-wide binder selection is the limited supply of suitable target antigens representative of the human proteome. Here, we provide proof of principle of using easily accessible, cancer-associated protein epitope signature tags (PrESTs), routinely generated within the Human Protein Atlas project, as surrogate antigens in phage selectionsfor the retrieval of target specific binders. These binders were subsequently tested in western blot, immunohistochemistry and protein microarray application to demonstrate their functionality.
Place, publisher, year, edition, pages
2011. Vol. 28, no 4, 302-311 p.
PrEST, monoclonal antibodies, phage display
IdentifiersURN: urn:nbn:se:kth:diva-25857DOI: 10.1016/j.nbt.2010.12.005ISI: 000292717500002PubMedID: 21232647ScopusID: 2-s2.0-79958056952OAI: oai:DiVA.org:kth-25857DiVA: diva2:360252
FunderScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Updated from submitted to published.2010-11-022010-11-022011-12-07Bibliographically approved