New Method for Fabrication of Fused Silica Emitters with Submicrometer Orifices for Nanoelectrospray Mass Spectrometry
2011 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 83, no 20, 7771-7777 p.Article in journal (Refereed) Published
In this paper, we describe a new method for fabrication of nanoelectrospray emitters. The needles were pulled from fused silica capillary tubing, which was melted by means of a plasma, formed by electrical discharges between two pointed platinum electrodes. A key feature of the pulling device is a rotating configuration of the electrodes, which results in an even radial heating of the capillary. The construction of the setup is straightforward, and needles with a variety of shapes can be fabricated, including orifices of submicrometer dimensions. Pulled needles with long tapered tips and an orifice of 0.5 mu m were utilized for electrospray ionization mass spectrometry (ESI-MS) of discrete sample volumes down to 275 pL. The picoliter-sized samples were transferred into the tip of the needle from a silicon microchip by aspiration. To avoid a rapid evaporation of the sample, all manipulations were performed under a cover of a fluorocarbon liquid. The limit of detection was measured to be ca. 20 attomole for insulin (chain B, oxidized).
Place, publisher, year, edition, pages
2011. Vol. 83, no 20, 7771-7777 p.
ELECTROSPRAY-IONIZATION SOURCE, DISCOVERY, NANOSPRAY
IdentifiersURN: urn:nbn:se:kth:diva-26072DOI: 10.1021/ac201308nISI: 000295817500028ScopusID: 2-s2.0-80054701334OAI: oai:DiVA.org:kth-26072DiVA: diva2:369689
QC 20101111 Updated from manuscript to article in journal.2010-11-112010-11-112011-11-07Bibliographically approved