Behavior of different monocomponent endoglucanases on the accessibility and reactivity of dissolving-grade pulps for viscose process
2010 (English)In: Enzyme and microbial technology, ISSN 0141-0229, E-ISSN 1879-0909, Vol. 47, 355-362 p.Article in journal (Refereed) Published
Three different commercial monocomponent endoglucanases, with and without a cellulose-binding domain (CBD) and differences in their glycosidic hydrolysis mechanisms, were compared with respect to their ability to enhance the accessibility and reactivity of dissolving-grade pulps for viscose production. Hardwood (eucalyptus) and softwood (mixture of Norway spruce and Scots pine) commercial dried and never-dried bleached sulfite dissolving pulps were used for this purpose. The effects of the enzymatic treatments on pulps were studied by reactivity, according to Fock's method, and viscosity measurements, and recording of molecular weight distributions. Among the different assayed enzymes, endoglucanase with a CBD and an inverting hydrolysis mechanism was found to be the most effective in increasing the reactivity of both pulps. Simultaneously, the viscosity decreased, being more marked for softwood dissolving pulp. A narrower molecular weight distribution, with a great reduction in the amount of long-chain cellulose molecules was observed in both pulps, being more pronounced for softwood dissolving pulp. By contrast, endoglucanase without a CBD and a retaining hydrolysis mechanism showed a barley enhancement of the studied properties. The effects of the different endoglucanase treatments were more pronounced when never-dried dissolving pulps were used.
Place, publisher, year, edition, pages
2010. Vol. 47, 355-362 p.
Hardwood, Softwood, Dissolving-grade pulp, Endoglucanase (EG), Cellulose-binding domain (CBD), Reactivity, Viscosity, Molecular weight distribution (MWD)
Paper, Pulp and Fiber Technology
IdentifiersURN: urn:nbn:se:kth:diva-26984DOI: 10.1016/j.enzmictec.2010.07.016ISI: 000284346000008ScopusID: 2-s2.0-77957868876OAI: oai:DiVA.org:kth-26984DiVA: diva2:373634
QC 201012012010-12-012010-12-012011-11-07Bibliographically approved