Ab initio structure determination from electron microscopic images of single molecules coexisting in different functional states
2010 (English)In: Structure, ISSN 0969-2126, E-ISSN 1878-4186, Vol. 18, no 7, 777-786 p.Article in journal (Refereed) Published
We have developed methods for ab initio three-dimensional (3D) structure determination from projection images of randomly oriented single molecules coexisting in multiple functional states, to aid the study of complex samples of macromolecules and nanoparticles by electron microscopy (EM). New algorithms for the determination of relative 3D orientations and conformational state assignment of single-molecule projection images are combined with well-established techniques for alignment and statistical image analysis. We describe how the methodology arrives at homogeneous groups of images aligned in 3D and discuss application to experimental EM data sets of the Escherichia coli ribosome and yeast RNA polymerase II.
Place, publisher, year, edition, pages
2010. Vol. 18, no 7, 777-786 p.
Proteins, RNA-POLYMERASE-II, CRYO-EM REVEALS, COMMON-LINES, 3-DIMENSIONAL RECONSTRUCTION, CONFORMATIONAL FLEXIBILITY, ORIENTATION REFINEMENT, BIOLOGICAL PARTICLES, 3-D RECONSTRUCTION, PROJECTIONS, RESOLUTION
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:kth:diva-27194DOI: 10.1016/j.str.2010.06.001ISI: 000280046300005PubMedID: 20637414ScopusID: 2-s2.0-77954629363OAI: oai:DiVA.org:kth-27194DiVA: diva2:375735
FunderSwedish Research Council
QC 201012092010-12-092010-12-092011-02-08Bibliographically approved