Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles
2010 (English)In: Journal of nanoparticle research, ISSN 1388-0764, E-ISSN 1572-896X, Vol. 12, no 8, 3057-3067 p.Article in journal (Refereed) Published
In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.
Place, publisher, year, edition, pages
2010. Vol. 12, no 8, 3057-3067 p.
Invertase, Immobilization, Dendrimer, SPION, Nanoparticle, Nanobiotechnology
Engineering and Technology
IdentifiersURN: urn:nbn:se:kth:diva-27366DOI: 10.1007/s11051-010-9902-9ISI: 000284275600036ScopusID: 2-s2.0-77955589344OAI: oai:DiVA.org:kth-27366DiVA: diva2:377357
QC 201012142010-12-142010-12-132010-12-14Bibliographically approved