Fluorescence-based transient state monitoring for biomolecular spectroscopy and imaging
2010 (English)In: Journal of the Royal Society Interface, ISSN 1742-5689, E-ISSN 1742-5662, Vol. 7, no 49, 1135-1144 p.Article, review/survey (Refereed) Published
To increase read-out speed, sensitivity or specificity, an often applied strategy in fluorescence-based biomolecular spectroscopy and imaging is to simultaneously record two or more of the fluorescence parameters: intensity, lifetime, polarization or wavelength. This review highlights how additional, to-date largely unexploited, information can be extracted by monitoring long-lived, photo-induced transient states of organic dyes and their dynamics. Two major approaches are presented, where the transient state information is obtained either from fluorescence fluctuation analysis or by recording the time-averaged fluorescence response to a time-modulated excitation. The two approaches combine the detection sensitivity of the fluorescence signal with the environmental sensitivity of the long-lived transient states. For both techniques, proof-of-principle experiments are reviewed, and advantages, limitations and possible applications for biomolecular cellular biology studies are discussed.
Place, publisher, year, edition, pages
London: Royal Society Publishing , 2010. Vol. 7, no 49, 1135-1144 p.
fluorescence, transient state imaging, triplet state, trans-cis isomerization, modulated excitation
Physical Sciences Biophysics Physical Chemistry
Research subject Biological Physics
IdentifiersURN: urn:nbn:se:kth:diva-27267DOI: 10.1098/rsif.2010.0146ISI: 000279180600001ScopusID: 2-s2.0-77955640586OAI: oai:DiVA.org:kth-27267DiVA: diva2:378723
FunderEU, European Research Council, FLUODIAMON 201837Swedish Research Council, VR-NT 2009-3134
QC 201012162010-12-162010-12-092016-03-10Bibliographically approved