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Microbead-based rolling circle amplification in a microchip for sensitive DNA detection
KTH, School of Biotechnology (BIO), Proteomics.
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2010 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 10, no 10, 1262-1266 p.Article in journal (Refereed) Published
Abstract [en]

The sensitive detection and quantification of DNA targets in the food industry and in environmental and clinical settings are issues of utmost importance in ensuring contamination-free food, monitoring the environment, and battling disease. Selective probes coupled with powerful amplification techniques are therefore of major interest. In this study, we set out to create an integrated microchemical chip that benefits from microfluidic chip technology in terms of sensitivity and a strong detection methodology provided jointly by padlock probes and rolling circle amplification (RCA). Here, we have integrated padlock probes and RCA into a microchip. The chip uses solid phase capture in a microchannel to enable washing cycles and decrease analytical area, and employs on-bead RCA for single-molecule amplification and detection. We investigated the effects of reagent concentration and amount of padlock probes, and demonstrated the feasibility of detecting Salmonella.

Place, publisher, year, edition, pages
2010. Vol. 10, no 10, 1262-1266 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-27902DOI: 10.1039/b927460jISI: 000277359600006Scopus ID: 2-s2.0-77951898837OAI: oai:DiVA.org:kth-27902DiVA: diva2:383053
Note
QC 20110104Available from: 2011-01-04 Created: 2011-01-03 Last updated: 2017-12-11Bibliographically approved

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