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Transient state microscopy: a new tool for biomolecular imaging
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.ORCID iD: 0000-0003-3200-0374
2009 (English)In: MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES IX / [ed] Periasamy A, So PTC, Bellingham, WA: SPIE-INT SOC OPTICAL ENGINEERING , 2009, Vol. 7183Conference paper, Published paper (Refereed)
Abstract [en]

Photoinduced transient dark states are exhibited by practically all common fluorophores. However, their information content has to date only been sparsely exploited due to methodological constraints. Here, a new concept is presented and verified that can monitor and image these states via their photodynamic fingerprints. It unites the environmental sensitivity of these states with the sensitivity of fluorescence-based detection. For demonstration, triplet state images of liposomes in different environments were generated, showing how local environmental differences can be resolved, not clearly distinguishable via other fluorescence parameters. The concept can provide several new, useful and independent fluorescence-based parameters in biomolecular imaging.

Place, publisher, year, edition, pages
Bellingham, WA: SPIE-INT SOC OPTICAL ENGINEERING , 2009. Vol. 7183
Series
Proceedings of SPIE-The International Society for Optical Engineering, ISSN 0277-786X ; 7183
Keyword [en]
modulated excitation, laser scanning microscopy, transient state imaging, triplet state imaging, oxygen monitoring
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-27956DOI: 10.1117/12.814908ISI: 000284822400062Scopus ID: 2-s2.0-65949086796ISBN: 978-0-8194-7429-2 (print)OAI: oai:DiVA.org:kth-27956DiVA: diva2:383120
Conference
Conference on Multiphoton Microscopy in the Biomedical Sciences IX San Jose, CA, JAN 25-27, 2009
Note
QC 20110104Available from: 2011-01-04 Created: 2011-01-03 Last updated: 2011-01-04Bibliographically approved

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CiteExportLink to record
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