Perfluorophenyl Azide Immobilization Chemistry for Single Molecule Force Spectroscopy of the Concanavalin A/Mannose Interaction
2010 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 26, no 22, 16677-16680 p.Article in journal (Refereed) Published
The versatility of perfluorophenyl azide (PFPA) derivatives makes them useful for attaching a wide variety of biomoleculcs and polymers to surfaces. Herein, it single molecule force spectroscopy (SMFS) study of the concanavalin A/mannose interaction was carried out using PFPA immobilization chemistry. SMFS of the concanavalin A/mannose interaction yielded in average unbinding force of 70-80 pN for loading rates between 8000 and 40 000 pN/s for mannose surfaces on aminated glass. and an unbinding force of 57 +/- 20 pN at 6960 pN/s for mannose surfaces on gold-coated glass. Dynamic force spectroscopy was used to determine the dissociation rate constant, k(off), for this interaction to be 0.16 s(-1).
Place, publisher, year, edition, pages
2010. Vol. 26, no 22, 16677-16680 p.
microscopy, cells, adhesion, bond
IdentifiersURN: urn:nbn:se:kth:diva-27946DOI: 10.1021/la1036579ISI: 000283837800012ScopusID: 2-s2.0-78650381981OAI: oai:DiVA.org:kth-27946DiVA: diva2:383223
QC 201101042011-01-042011-01-032011-10-04Bibliographically approved