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Perfluorophenyl Azide Immobilization Chemistry for Single Molecule Force Spectroscopy of the Concanavalin A/Mannose Interaction
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
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2010 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 26, no 22, 16677-16680 p.Article in journal (Refereed) Published
Abstract [en]

The versatility of perfluorophenyl azide (PFPA) derivatives makes them useful for attaching a wide variety of biomoleculcs and polymers to surfaces. Herein, it single molecule force spectroscopy (SMFS) study of the concanavalin A/mannose interaction was carried out using PFPA immobilization chemistry. SMFS of the concanavalin A/mannose interaction yielded in average unbinding force of 70-80 pN for loading rates between 8000 and 40 000 pN/s for mannose surfaces on aminated glass. and an unbinding force of 57 +/- 20 pN at 6960 pN/s for mannose surfaces on gold-coated glass. Dynamic force spectroscopy was used to determine the dissociation rate constant, k(off), for this interaction to be 0.16 s(-1).

Place, publisher, year, edition, pages
2010. Vol. 26, no 22, 16677-16680 p.
Keyword [en]
microscopy, cells, adhesion, bond
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-27946DOI: 10.1021/la1036579ISI: 000283837800012Scopus ID: 2-s2.0-78650381981OAI: oai:DiVA.org:kth-27946DiVA: diva2:383223
Note
QC 20110104Available from: 2011-01-04 Created: 2011-01-03 Last updated: 2011-10-04Bibliographically approved
In thesis
1. Photochemical Surface Functionalization: Synthesis, Nanochemistry and Glycobiological Studies
Open this publication in new window or tab >>Photochemical Surface Functionalization: Synthesis, Nanochemistry and Glycobiological Studies
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis mainly deals with the development of photochemical approaches to immobilize carbohydrates on surfaces for glycobiological studies. These approaches have been incorporated into a number of state-of-the-art nanobio-platforms, including carbohydrate microarrays, surface plasmon resonance (SPR), quartz crystal microbalance (QCM), atomic force microscopy (AFM), and glyconanomaterials. All the surfaces have displayed good binding capabilities and selectivities after functionalization with carbohydrates, and a range of important data have been obtained concerning surface characteristics and carbohydrate-protein interactions, based on the platforms established. Besides, a variety of non-carbohydrate and carbohydrate-based molecules have been synthesized, during which process the mutarotation of 1-glycosyl thiols and the stereocontrol in 1-S-glycosylation reactions have been thoroughly studied.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2011. 86 p.
Series
Trita-CHE-Report, ISSN 1654-1081 ; 2011:47
Keyword
Carbohydrates, Glycobiology, Carbohydrate-protein interactions, Perfluorophenylazide (PFPA), Photochemistry, Carbohydrate surface immobilization, Carbohydrate microarrays, Surface plasmon resonance (SPR), Quartz crystal microbalance (QCM), Atomic force microscopy (AFM), Glyconanomaterials, Concanavalin A (Con A), Cyanovirin-N (CV-N), 1-Glycosyl thiols; Mutarotation, 1-S-glycosylation, Stereocontrol
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-41973 (URN)978-91-7501-072-4 (ISBN)
Public defence
2011-10-21, F3, Lindstedtsvägen 26, KTH, Stockholm, 13:00 (English)
Opponent
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Note
QC 20111004Available from: 2011-10-04 Created: 2011-10-04 Last updated: 2011-10-04Bibliographically approved

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