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Creation of an antibody-based subcellular protein atlas
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0001-7034-0850
KTH, School of Biotechnology (BIO), Proteomics.ORCID iD: 0000-0001-8993-048X
2010 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 10, no 22, 3984-3996 p.Article, review/survey (Refereed) Published
Abstract [en]

An important part for understanding the complex machinery of living cells is to know the spatial distribution of proteins all the way from organ to organelle levels. An equally important part of proteomics is to map the subcellular distribution of all human proteins. Here, we discuss methodologies for systematic subcellular profiling with emphasis on the antibody-based approach performed as a part of the Human Protein Atlas project. The considerations made when creating the subcellular protein atlas and critical parameters of this approach are discussed.

Place, publisher, year, edition, pages
2010. Vol. 10, no 22, 3984-3996 p.
Keyword [en]
Antibody, Cell biology, Confocal microscopy, Human Protein Atlas, Organelle, Subcellular profiling
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-28181DOI: 10.1002/pmic.201000125ISI: 000285164500005ScopusID: 2-s2.0-78449277726OAI: diva2:385234
Knut and Alice Wallenberg FoundationEU, FP7, Seventh Framework Programme
QC 20110111Available from: 2011-01-11 Created: 2011-01-10 Last updated: 2011-01-11Bibliographically approved

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