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Glyconanomaterials: Synthesis, Characterization, and Ligand Presentation
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Organic Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry.ORCID iD: 0000-0003-1121-4007
2010 (English)In: Advanced Materials, ISSN 0935-9648, E-ISSN 1521-4095, Vol. 22, no 17, 1946-1953 p.Article in journal (Refereed) Published
Abstract [en]

Glyconanomaterials, nanomaterials carrying surface-tethered carbohydrate ligands, have emerged and demonstrated increasing potential in biomedical imaging, therapeutics, and diagnostics. These materials combine the unique properties of nanometer-scale objects with the ability to present multiple copies of carbohydrate ligands, greatly enhancing the weak affinity of individual ligands to their binding partners. Critical to the performance of glyconanomaterials is the proper display of carbohydrate ligands, taking into consideration of the coupling chemistry, the type and length of the spacer linkage, and the ligand density. This article provides an overview of the coupling chemistry for attaching carbohydrate ligands to nanomaterials, and discusses the need for thorough characterization of glyconanomaterials, especially quantitative analyses of the ligand density and binding affinities. Using glyconanoparticles synthesized by a versatile photocoupling chemistry, methods for determining the ligand density by colorimetry and the binding affinity with lectins by a fluorescence competition assay are determined. The results show that the multivalent presentation of carbohydrate ligands significantly enhances the binding affinity by several orders of magnitude in comparison to the free ligands in solution. The effect is sizeable even at low surface ligand density. The type and length of the spacer linkage also affect the binding affinity, with the longer linkage promoting the association of bound ligands with the corresponding lectins.

Place, publisher, year, edition, pages
2010. Vol. 22, no 17, 1946-1953 p.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-27865DOI: 10.1002/adma.200903908ISI: 000277949200011Scopus ID: 2-s2.0-77951961399OAI: oai:DiVA.org:kth-27865DiVA: diva2:385878
Note

QC 20150728

Available from: 2011-01-12 Created: 2011-01-03 Last updated: 2017-12-11Bibliographically approved

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