Change search
ReferencesLink to record
Permanent link

Direct link
Biosynthesis of Callose and Cellulose by Detergent Extracts of Tobacco Cell Membranes and Quantification of the Polymers Synthesized in vitro
KTH, School of Biotechnology (BIO), Glycoscience.
2010 (English)In: J INTEGR PLANT BIOL, ISSN 1672-9072, Vol. 52, no 2, 221-233 p.Article in journal (Refereed) Published
Abstract [en]

The conditions that favor the in vitro synthesis of cellulose from tobacco BY-2 cell extracts were determined. The procedure leading to the highest yield of cellulose consisted of incubating digitonin extracts of membranes from 11-day-old tobacco BY-2 cells in the presence of 1 mM UDP-glucose, 8 mM Ca2+ and 8 mM Mg2+. Under these conditions, up to nearly 40% of the polysaccharides synthesized in vitro corresponded to cellulose, the other polymer synthesized being callose. Transmission electron microscopy analysis revealed the occurrence of two types of structures in the synthetic reactions. The first type consisted of small aggregates with a diameter between 3 and 5 nm that associated to form fibrillar strings of a maximum length of 400 nm. These structures were sensitive to the acetic/nitric acid treatment of Updegraff and corresponded to callose. The second type of structures was resistant to the Updegraff reagent and corresponded to straight cellulose microfibrils of 2-3 nm in diameter and 200 nm to up to 5 mu m in length. In vitro reactions performed on electron microscopy grids indicated that the minimal rate of microfibril elongation in vitro is 120 nm/min. Measurements of retardance by liquid crystal polarization microscopy as a function of time showed that small groups of microfibrils increased in retardance by up to 0.047 nm/min per pixel, confirming the formation of organized structures.

Place, publisher, year, edition, pages
2010. Vol. 52, no 2, 221-233 p.
National Category
Plant Biotechnology Industrial Biotechnology Biochemistry and Molecular Biology
URN: urn:nbn:se:kth:diva-28411DOI: 10.1111/j.1744-7909.2010.00919.xISI: 000276034000010ScopusID: 2-s2.0-77749264219OAI: diva2:388779
QC 20110118Available from: 2011-01-18 Created: 2011-01-14 Last updated: 2011-01-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Bulone, Vincent
By organisation
Plant BiotechnologyIndustrial BiotechnologyBiochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link