Affibody molecule-mediated depletion of HSA and IgG using different buffer compositions: a 15 min protocol for parallel processing of 1-48 samples
2010 (English)In: Biotechnology and applied biochemistry, ISSN 0885-4513, E-ISSN 1470-8744, Vol. 56, 49-57 p.Article in journal (Refereed) Published
High-abundant plasma proteins pose a challenge in a large number of proteomics-based technologies. Depletion of these high-abundant proteins has proven to be a fruitful strategy to circumvent masking of lower-abundant proteins that could serve as valuable biomarker candidates. However, current strategies often do not meet the throughput requirements of large-scale proteomic studies. In the present paper, a flexible and parallelized method for the depletion of high-abundant proteins is described, allowing the removal of the two most abundant proteins from 48 blood-derived samples in less than 15 min using Affibody molecules as affinity ligands. A sample-processing platform like this should be suitable for a number of proteomics technologies; its flexibility in buffer composition allows for different types of downstream applications.
Place, publisher, year, edition, pages
2010. Vol. 56, 49-57 p.
Affibody molecule, human serum albumin (HSA), IgG, protein depletion, high throughput analysis, sample preparation, serum or plasma analysis
IdentifiersURN: urn:nbn:se:kth:diva-29471DOI: 10.1042/BA20100041ISI: 000280262400002ScopusID: 2-s2.0-77954833249OAI: oai:DiVA.org:kth-29471DiVA: diva2:395828
FunderSwedish Research CouncilKnut and Alice Wallenberg Foundation
QC 201102082011-02-082011-02-022011-02-08Bibliographically approved